1) Investigación >
Artículos Publicados >

Por favor, use este identificador para citar o enlazar este ítem:

Título : In vitro levels of cytokines in response to purified protein derivative (PPD) antigen in a population with high prevalence of pulmonary tuberculosis
Autor : Giampietro, Francesca
De Waard, Jacobus H.
Rivas- Santiago, Bruno
Encimo- Moreno, Jose Antonio
Salgado, Antonio
Araujo, Zaida
Palabras clave : TB
Fecha de publicación : Jul-2010
Editorial : Human Immunology
Citación : vol. 71;
Resumen : The control of mycobacterial infection by the host depends on cell-mediated immunity (CMI), involving activated macrophages, T cells, and type 1 cytokines (Th1). Here we evaluated the capacity of antigeninduced proliferation by peripheral blood mononuclear cells (PBMCs) and the production of proinflammatory tumor necrosis factor-alpha (TNF- ) and interleukin 12 (IL-12p40). The Th1 cytokine interferon-gamma (IFN– ) and Th2 cytokines interleukin 4 (IL-4) and interleukin 5 (IL-5) in 62 pulmonary tuberculosis (TB) patients (40 Warao indigenous patients [WP] and 22 Creole nonindigenous patients [CP]) and 24 healthy controls (12 Warao indigenous controls [WC] and 12 Creole nonindigenous controls [CC]) at 24 and 48 hours in response to purified protein derivative (PPD) from Mycobacterium tuberculosis. The overall results revealed that testing of CP and CC’ PBMCs for TNF- , IFN- , and IL-12p40 production was higher compared with WP and WC’ PBMCs after stimulating for 24 and 48 hours (p 0.0001), within the WP group, the lower productions of IL-12p40 and IFN- significantly correlated (r2 0.91, p 0.01). Although in general there was interindividual variability in the observed responses of Th2 cytokines, especially with IL-4, there was a trend to produce higher PPD-induced IL-5 by WP’ PBMCs compared with WC’ PBMCs and CP’ PBMCs at 24 and 48hours, respectively. High IL-5 production correlated inversely with low IFN- production (r2 0.97, p 0.002). In conclusion, our results suggest that PPD-induced responses observed in patients from both populations can be divided into two groups: one group that activates Creole’ PBMCs to preferentially secrete TNF- , IL-12p40 and IFN- and another group that activates preferential secretion of IL-5 in Warao’ PBMCs.2010 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
ISSN : 1099–1104
Aparece en las colecciones: Artículos Publicados

Ficheros en este ítem:

Fichero Descripción Tamaño Formato
HIM.Cytokines.2010.pdf469.33 kBAdobe PDFVisualizar/Abrir

Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2008 MIT and Hewlett-Packard - Comentarios