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Título : The role of a H+-ATPase in the regulation of cytoplasmic pH in Trypanosoma cruzi epimastigotes
Autor : VANDERHEYDEN, Nicole
BENAÍM, Gustavo
DOCAMPO, Roberto
Palabras clave : Cytoplasmic pH
Trypanosoma cruzi
omeprazole
vanadate
bafilomycin A1
fluorescent probe bisoxonol
Fecha de publicación : 1996
Editorial : The Biochemical journal
Citación : Vol. 318;pp 103-109
Resumen : Cytoplasmic pH (pHi) regulation was studied in Trypanosoma cruzi epimastigotes using fluorescent probes. Steady-state pH1 was maintained even in the absence of extracellular Na+ or K+, but was significantly decreased in the absence of C1−. Acidloaded epimastigotes regained normal pH1 by a process that was ATP-dependent and sensitive to N-ethylmaleimide, dicyclohexylcarbodi- imide and diethylstilboestrol, suggesting involvement of a H+-pumping ATPase. Recovery from an acid load was independent of extracellular Na+ or K+ and insensitive to omeprazole, vanadate and low concentrations of bafilomycin A1. Using the fluorescent probe bisoxonol to measure the membrane potential of intact cells, acid loading of epimastigotes was shown to result in a dicyclohexylcarbodi-imide-sensitive hyperpolarization, which suggests electrogenic pumping of protons across the plasma membrane. Addition of glucose, but not of 6- deoxyglucose, produced a transient cellular acidification of possible metabolic origin, and increased the rate of recovery from an acid load. Taken together, these results are consistent with an important role of a H+-ATPase in the regulation of pH1 homoeostasis in T. cruzi.
Descripción : Department of Veterinary Pathobiology, University of Illinois at Urbana-Champaign, 2001 S. Lincoln Ave., Urbana, IL 61801, U.S.A.
URI : http://hdl.handle.net/10872/15708
ISSN : 1470-8728 (Electronic)
0264-6021 (Linking)
0264-6021 (Print)
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