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Título : The plasma membrane Ca2+-ATPase protein from red blood cells is not modified in preeclampsia
Autor : Oviedo, Néstor J.
Benaím, Gustavo
Cervino, Vincenza
Proverbio, Teresa
Proverbio, Fulgencio
Marín, Reinaldo
Palabras clave : Preeclampsia
Human red blood cell
Lipid peroxidation
Plasma membrane
Fecha de publicación : 2006
Editorial : Biochimica et Biophysica Acta
Citación : Vol. 1762;No. 3 pp 381–385
Resumen : Plasma membrane Ca2+-ATPase activity diminishes by about 50% in red blood cells during preeclampsia. We investigated whether the number of Ca2+-ATPase molecules is modified in red cell membranes from preeclamptic pregnant women by measuring the specific phosphorylated intermediate of this enzyme. Also, we isolated the Ca2+-ATPase protein from both normotensive and preeclamptic pregnant women and estimated its molecular weight, and its cross-reactions with specific polyclonal and monoclonal (5F10) antibodies against it. We measured the Ca2+-ATPase activity in a purified state and the effect of known modulators of this ATPase. It was found that the phosphorylated intermediate associated with PMCA is similar for red cell ghosts from normotensive and preeclamptic women, suggesting a similar number of ATPase molecules in these membranes. The molecular weight of the Ca2+-ATPase is around 140 kDa for both normotensive and preeclamptic membranes, and its crossreactions with specific antibodies is similar, suggesting that the protein structure remains intact in preeclampsia. Calmodulin, ethanol, or both calmodulin plus ethanol, stimulated the Ca2+-ATPase activity to the same extent for both normotensive and preeclamptic preparations. Our results showed that the reduced Ca2+-ATPase activity of the red cell membranes from preeclamptic women is not associated with a defective enzyme, but rather with a high level of lipid peroxidation.
Descripción : Corresponding author. Fax: +58 212 504 1093. E-mail address: (R. Marín). Current address: Department of Cytokine Biology, The Forsyth Institute and Department of Developmental Biology, Harvard School of Medicine, Boston, MA 02115, USA.
ISSN : 0925-4439
1878-2434 (Electronic)
0006-3002 (Linking)
0006-3002 (Print)
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